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The exocyst gene Sec10 regulates renal epithelial monolayer homeostasis and apoptotic sensitivity.

Highly conserved protein complex exocyst Organize polarized exocytosis of subsets of secretory vesicles. Previous research reported that shRNA knockdown of exocyst subunit center, Sec10 (Sec10-KD) in Madin-Darby canine kidney (MDCK) cells undisturbed primary cilia assembly and the formation of cysts in 3D. We use a three-dimensional collagen Mouse Clia Kits  culture of MDCK cells to further investigate the mechanisms governing exocyst Sec10 and polarity of epithelial morphogenesis and homeostasis. Sec10-KD cyst initially showed lumen formation bothered though later shown to significantly fewer and shorter primary cilia of control. 

Then in cystogenesis, control cells maintained normal homeostasis, whereas cyst Sec10-KD displayed many apoptotic cells basally extruded into the collagen matrix. Sec10-KD MDCK cells are also more sensitive to triggers of apoptosis compared with the control. This phenotype is reversed by restoring the expression of the human Sec10 Sec10 shRNA-resistant. Apico-basal polarity appears normal in Sec10-KD cyst, while the mitotic spindle angle significantly different from the control, showing planar cell polarity defects. 

In addition, analysis of the renal tubules in mouse models Sec10-KO generated new specific kidney revealed significant defects in primary cilia in kidney tubules assembly and targeted; abnormal epithelial cell extrusion is also observed, supporting our in vitro results. We hypothesize that, in Sec10-KD cells, disrupted exocyst activity results increased sensitivity to apoptosis through the primary signal and defective cilia which, in combination with increased levels of basal cell extrusion, it affects the epithelial barrier integrity and homeostasis.
The exocyst gene Sec10 regulates renal epithelial monolayer homeostasis and apoptotic sensitivity.

A probe for the detection of colorimetric lead ions (Pb (2+)) has been designed with a newly synthesized using a clip-like cyanine dyes and G-quadruplex. The unique structure cyanine dye clip-like probe is endowed with a high selectivity towards Pb (2+). Significant changes in the absorption spectrum of cyanine dye admit Pb (2 +) - induced conformational transition of G-quadruplexes make probes show high sensitivity to Pb (2+) with a detection limit of 1nm. The excellent performance allows the probe to be practically applied in measuring Pb (2+) pollution in Ovine Clia Kits freshwater systems.

Highly conserved protein complex exocyst Organize polarized exocytosis of subsets of secretory vesicles. Previous research reported that shRNA knockdown of exocyst center subunit, Sec10 (Sec10-KD) in Madin-Darby canine kidney (MDCK) cells undisturbed primary cilia assembly and the formation of cysts in 3D. We use a three-dimensional collagen culture of MDCK cells to further investigate the mechanisms that govern exocyst Sec10 and polarity of epithelial morphogenesis and homeostasis. Sec10-KD cyst lumen formation initially showed disrupted even though later proven to significantly fewer and shorter primary cilia control. Then in cystogenesis, control cells maintained normal homeostasis, whereas cyst Sec10-KD displayed many apoptotic cells basally extruded into the collagen matrix. Sec10-KD MDCK cells are also more sensitive to triggers apoptosis compared with controls. 

This phenotype is reversed by restoring human expression Sec10 Sec10 shRNA-resistant. Apico-basal polarity seems normal in Sec10-KD cyst, while the mitotic spindle angle significantly different from the controls, indicating the planar cell polarity defects. In addition, analysis of the renal tubules in mouse models generated Sec10-KO certain kidney newly revealed significant defects in primary cilia in kidney tubules assembly and targeted; abnormal epithelial cell extrusion is also observed, supporting our in vitro results. We hypothesize that, in Sec10-KD cells, disrupted exocyst activity results increased sensitivity to apoptosis through the primary signaling and cilia defects which, in combination with increased levels of basal cell extrusion, it affects the epithelial barrier integrity and homeostasis.

Investigation for the detection of colorimetric lead ions (Pb (2+)) has been designed with the newly synthesized using a clip-like cyanine dyes and G-quadruplex. The unique structure cyanine dye clip-like probe is endowed with a high selectivity towards Pb (2+). Significant changes in the absorption spectrum of cyanine dye admit Pb (2 +) - induced conformational transition of G-quadruplexes make probes show high sensitivity to Pb (2+) with a detection limit of 1nm. The excellent performance allows the probe to be practically applied in measuring Pb (2+) pollution in freshwater systems.
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